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Stem cell lines

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 Stem cell lines are developed elsewhere and usually sent for chromosome analysis as attached semiconfluent cultures in T75 flasks.

 Upon arrival, all transport medium is removed and replaced with 10 ml regular cell culture medium (specified by the stem cell laboratory).

 The cells are grown at 37°C in 5% CO2, inspected every day, and harvested when many mitotic cells are visible (Figure 22.1).

Equine Reproductive Procedures

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