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 Glass slides for chromosome preparations must be precleaned by soaking overnight in glass cleaning solution (chromic‐sulphuric acid) followed by overnight rinsing under running tap water. Clean slides are stored in distilled H2O at 4°C (39°F) until needed. A slide is clean when a water film spreads evenly over the glass surface.

 To make chromosome preparations, drop approximately 10 μl of fixed cell suspension on one end of a wet slide and allow it to spread on the slide. Let the slide air‐dry and check it under a light microscope using 20× phase contrast objective for the number and quality of metaphase spreads. If the number of spreads is low, the cell suspension should be concentrated by centrifugation. If the metaphases are surrounded by cytoplasm and chromosomes are not spread, wash the cells with fixative for 2–3 more times. Slides can be stored in air‐tight boxes containing desiccant at –20°C for several years.