Читать книгу Pancreatic Tumors - Группа авторов - Страница 35

Adequate smear preparation is critical to accurate interpretation. Techniques for adequate smear preparation have been well described in the cytology literature [1]. A good-quality smear shows the cellular material evenly and thinly spread out on the slide (Fig. 1). Factors causing difficulty in smear interpretation include a bloody smear, a thick smear, and crush artifact (Fig. 2). It is recommended that large clots or tissue fragments be lifted from the smear and submitted for cell block analysis. Thick tissue fragments or clots will not be well visualized on smears.

Fig. 1. A well-spread smear – the aspirated material is evenly smeared making it easy to assess the cytological features of the cells. Diff-Quik stain, ×10.

Fig. 2. A thick smear – the aspirated material is entangled in a blood clot making it difficult to assess the cytological features of the cells. Diff-Quik stain, ×10.