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Performing ROSE

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A staff member from the clinical team/cytotechnologist/cytopathologist assists in specimen collection during the procedure and with smear preparation in the procedure room or suite. At our institution two slides are prepared from each pass. One slide is air dried for Diff-Quik staining and the second slide is fixed in 95% alcohol for later Papanicolaou (PAP) staining in the laboratory. The needle is rinsed in balanced salt solution for cell block processing. The Diff-Quik-stained slide is used for on-site morphological evaluation of adequacy. Additional needle passes are performed based on the assessment of the Diff-Quik-stained smears, and material collected for additional ancillary studies based on the on-site adequacy.

Pancreatic Tumors

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