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Collection Media
ОглавлениеIf ancillary studies are needed, additional dedicated passes for cell block material are made. Different types of collection mediums, such as saline, Roswell Park Memorial Institute (RPMI), neutral-buffered formalin, a mixture of formalin and 95% ethanol, and methanol, are available and can be utilized for needle rinses. Specimens collected in saline and RPMI can be used for flow cytometry if needed. Needle rinses can be processed into cell blocks or cytospin slides if the material collected is scant. Needle rinses collected in preservatives containing alcohol (methanol in PreservCyt and ethanol in SurePath Preservative Fluid) can be processed using ThinPrep® and SurePathTM respectively. Although ThinPrep preparation is being used for pancreatic FNAs, there are certain pitfalls and limitations to it. It is difficult to recognize mucin on ThinPrep slides and mucinous neoplasms may be incorrectly diagnosed. A study by de Luna et al. [18] concluded that the diagnostic accuracy of pancreatic FNA using ThinPrep is inferior to that of conventional smears. However, ThinPrep® remains a viable substitute for smear preparation for practices in which a cytologist is not available on-site to prepare the slides. At our institution, we receive pancreatic FNA specimens submitted directly into CytoLyt, and have found them to have a good diagnostic yield. Aspirates form cysts have been identified to have background mucin and the epithelium is well preserved.