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Biocatalytic Processes for Biodiesel Production

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Ubaid Mehmood1, Faizan Muneer2, Muhammad Riaz3, Saba Sarfraz4 and Habibullah Nadeem2*

1College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, China

2Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Pakistan

3Department of Food Sciences, University College of Agriculture, Bahauddin Zakariya University, Multan, Pakistan

4Department of Chemistry, Government College Women University Faisalabad, Faisalabad, Pakistan

Abstract

Enzymes such as microbial lipases can be effectively used as biocatalysts for bio-diesel production in a sustainable manner. Biocatalytic processes to produce bio-diesel or biofuel is the need of time to reduce the emission of greenhouse gases produced from conventional diesel or fossil fuels. Lipases with excellent biochemical and physiological properties are most commonly used to catalyze the transesterification process for biodiesel production. Lipases obtained from microbes such as bacteria and fungi produce 70%–95% ethanol and methanol. Biodiesel is usually composed of fatty acid alkyl esters which are mono-alkyl esters of either fatty acid methyl esters or fatty acid ethyl esters depending upon the alcohol (acyl acceptor) being used in the reaction. Factors such as bioreactor type, acyl acceptor, temperature, and glycerol can affect the enzymatic transesterification reaction. Recombinant enzymes such as recombinant lipases can be employed to obtain higher percentage of biodiesel due to their high specificity and biocatalytic activity for different substrates used for biodiesel production.

Keywords: Lipases, biodiesel, biocatalysis, biofuels, Novozyme, free fatty acids, ethyl acceptors

Biodiesel Technology and Applications

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