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There are a lot of factors effecting enzymatic transesterification reaction such as source of enzyme, its type, preparation method, applying technique, its dosage, activity, and life time. Apart from these enzymes related factors, there are also some other factors which affect transesterification reaction, e.g., feedstock type and its quality, type of alcohol as acyl acceptor, reaction pH, presence or absence of solvent, type of solvent, reaction temperature, alcohol-to-oil molar ratio [46].