Читать книгу Handbook of Intelligent Computing and Optimization for Sustainable Development - Группа авторов - Страница 56

Deoxyribozyme logic gate works as NOT gate if the inclusion of single stem-loop region modifies the catalytic core of the enzyme. It controls the enzyme activity by controlling the binding of substrate to the specific region of the enzyme. The mechanism of NOT gate is pictorially explained in Figure 2.15. The NOTz gate as shown in the figure is first in its active mode. If a single stranded input sequence iz is added to deoxyribosome, then it hybridizes to the input binding region (red) of the enzyme which leads to the deformation of the catalytic core. This conformational alteration of the enzyme structure inhibits the enzyme activity, i.e., inactivates the DNA logic gate. Thus, if the input iz is bound to the enzyme, then the substrate cannot be cleaved. Table 2.5 represents the truth table for NOT gate.