Читать книгу Genome Editing in Drug Discovery - Группа авторов - Страница 62

The CRISPR toolkit for genome editing has been rapidly evolving and next‐generation CRISPR technologies have been applied for genetic screening in vivo. For example, in vivo screens based on CRISPRa (Braun et al. 2016; Ebright et al. 2020), CRISPRi (Li et al. 2020), exon excision (Thomas et al. 2020), and Cas12a/Cpf1‐mediated double gene knockout (Chow et al. 2019) have been reported. Most recently, pooled in vivo CRISPR screening has been combined with single‐cell RNA‐seq analysis so that the relationships between genotypes to in vivo phenotypes can be mapped at single‐cell resolution (Giladi et al. 2018; Jaitin et al. 2016). We anticipate this approach to be coupled with diverse functional readouts across a broad spectrum of animal models and continue to transform biomedical research and drug discovery.