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For most experiments, CRISPR outsourcing falls into three broad categories: (i) CRISPR genetic screening; (ii) custom cell model generation; (iii) animal model generation. Animal model generation is out of scope for this chapter. For several commonly used cell lines, KO clones may already be commercially available, e.g. the human haploid HAP1 collection comprising over 7500 knockout cell lines. However, in the majority of cases, a custom edit will need to be generated. So far, we have provided a general description of the overall process of working with CROs. The remaining discussion will focus on specific aspects of the experimental design that are critical to discuss when outsourcing cell line generation or CRISPR genetic screens.

Figure 4.1 Process workflow for project externalization of genome editing to CROs.