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1.6 Conclusions and Future Prospects

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Nitric oxide is a dynamic molecule that performs a variety of physiological functions in both plants and animals. NO signaling plays a part in an array of plant biological functions, growth and developmental patterns, and abiotic and biotic stress tolerance. NO biosynthesis involves a number of complex mechanisms, including both enzymatic and nonenzymatic processes. It is necessary to conduct research on the substrates and inhibitors of the NO synthase enzyme, as well as its possible action on plants, in order to identify NO-generating enzymes. The association of NO with secondary signaling molecules as well as various phytohormones, and their possible mechanisms and pathways, need to be investigated further.

Future research should focus on the spatial and temporal distribution of NO in plants, as well as its intercellular and intracellular effects. In the future, the role of S-nitrosylation in the plant system and in ABA during the NO signaling mechanism must be recognized. Although the journey of NO research has been pushed back over the past few decades, a path forward must still be explored. Agriculture’s future requires effective research on the role of NO in increasing crop productivity and balancing ecological sustainability. The role of the NO and RNS-derived family in plant biology is being studied extensively. Elucidation of the role of NO in plant growth, seed germination, antioxidant defense mechanisms, postharvest harvest management, and the ripening phenomenon will open the door for future NO signaling research. As a result, much more research activity in NO studies is required to explore nitric oxide-mediated responses in the plant kingdom, as NO research opportunities are limitless and endless.

Nitric Oxide in Plants

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