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2.3.5 Phagokinetic Studies

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Albrecht-Buehler studied cell motility based upon observation of phagokinetic track, which can be described as a path generated by the cell as it passes over a layer of “biological markers” and engulfs them, leaving behind a halo (blank spot) equivalent to the area it has navigated [100]. This method provides a mechanism of studying and integrating the cell motility while preserving the history of individual paths. Opto-electrical methods can be used for studying these tracks [101]. Initially, the tracks were studied using the luminescence technique, which has very short-lived intensity as it quenches very fast. Colloidal quantum dots, with tunable light emitting properties, have emerged as a suitable alternative for biological labeling experiments wherein these colloidal semiconductor nanocrystals are spontaneously ingested by a wide variety of cells and remain intact within the cells for weeks enabling the tracking of phagokinetic tracks within cells [102].

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