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Biotechnology of Fruit and Nut Crops
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1. Introduction 1.1. Botany and history
1.2. Breeding and genetics
1.2.1. Rootstocks
1.2.2. Scions
2. Molecular Genetics 2.1. Marker-assisted selection
2.2. Gene cloning
2.3. Genomics
3. Micropropagation
4. Micrografting
5. Somatic Cell Genetics 5.1. Regeneration 5.1.1. Somatic embryogenesis
5.1.2. Organogenesis
5.1.3. Haploid recovery
5.1.4. Triploid recovery
5.1.5. Protoplast isolation and culture
5.2. Genetic manipulation 5.2.1. Mutation induction and somaclonal variation
5.2.2. Somatic hybridization
5.2.3. Genetic transformation
5.3. Cryopreservation
6. Conclusions
Acknowledgements
References
Страница 30
1. Introduction 1.1. Botany and history
1.2. Breeding and genetics
1.2.1. Rootstocks
1.2.2. Scions
2. Molecular Genetics 2.1. Marker-assisted selection
2.2. Gene cloning and proteomics
3. Micropropagation
4. Micrografting
5. Somatic Cell Genetics 5.1. Regeneration 5.1.1. Somatic embryogenesis
5.1.2. Organogenesis
5.2. Genetic manipulation 5.2.1. Somaclonal variation
5.2.2. Genetic transformation
6. Conclusions
Acknowledgements
References
Страница 46
1. Introduction
1.1. Botany and history
1.2. Importance
1.3. Breeding and genetics
1.3.1. Rootstocks
1.3.2. Scions
2. Molecular Genetics 2.1. Molecular markers 2.1.1. Protein markers
2.1.2. DNA markers
2.2. Gene mapping
2.3. Gene cloning
3. Micropropagation
4. Micrografting
5. Somatic Cell Genetics 5.1. Regeneration 5.1.1. Somatic embryogenesis
5.1.2. Organogenesis
5.1.3. Protoplast isolation and culture
5.2. Genetic manipulation 5.2.1. In vitro mutation induction and somaclonal variation
5.2.2. Genetic transformation
6.
In Vitro
Storage
7. Conclusions
References
Страница 67
1. Introduction 1.1. Botany and history
1.2. Breeding and genetics
1.2.1. Rootstocks
1.2.2. Scions
2. Molecular Genetics 2.1. Genetic diversity
2.2. Marker-assisted selection
2.3. Gene cloning
2.4. Genomics
3. Micropropagation
4. Micrografting
5. Somatic Cell Genetics 5.1. Regeneration 5.1.1. Somatic embryogenesis
5.1.2. Organogenesis
5.1.3. Protoplast isolation and culture
5.2. Genetic manipulation 5.2.1. Somaclonal variation
5.2.2. Genetic transformation
5.3. Cryopreservation
6. Conclusions
References
Страница 86
1. Introduction 1.1. Botany and history
1.2. Importance
1.2.1. Major breeding objectives
1.2.2. Breeding accomplishments
2. Molecular Genetics 2.1. Molecular markers
2.2. Genomics
3. Micropropagation
4. Somatic Cell Genetics 4.1. Regeneration 4.1.1. Organogenesis
4.1.2. Haploid recovery
4.1.3. Triploid recovery
4.1.4. Protoplast isolation and culture
4.2. Genetic manipulation
4.3. Cryopreservation
5. Conclusions
References
Страница 102
1. Introduction
1.1. Botany and history 1.1.1. Origin and history
1.1.2. General classification
1.1.3. Botanical description
1.2. Breeding and genetics 1.2.1. Breeding objectives
1.2.2. Conventional breeding
1.2.3. Current challenges and opportunities
2. Molecular Genetics
2.1. Marker-assisted selection (MAS)
2.2. Genomics and transcriptomics 2.2.1. Genomic data
2.2.2. Transcriptomic data
3. Somatic Cell Genetics
3.1. Regeneration 3.1.1. Somatic embryogenesis
3.1.2. Haploid recovery
3.1.3. Protoplast isolation and culture
3.2. Genetic manipulation 3.2.1. Mutation induction and somaclonal variation
3.2.2. Genetic transformation
3.3. Conservation 3.3.1. Short- and medium-term conservation of coconut germplasm
3.3.2. Long-term conservation via cryopreservation
4. Conclusions
Acknowledgements
References
Страница 125
1. Introduction
1.1. Botany and history
1.2. Breeding and genetics
1.2.1. Major breeding objectives
1.2.2. Breeding accomplishments
2. Molecular Genetics
2.1. Marker-assisted selection
2.2. Gene discovery 2.2.1. The SHELL gene, keystone of oil palm breeding
2.2.2. Fruit development
2.2.3. Embryo development
2.2.4. Oil biosynthesis
2.2.5. Lipid degradation
2.2.6. Fruit dehiscence
2.2.7. Defence response to diseases
2.2.8. Gender plasticity
2.2.9. DNA methylation
2.3. Genomics
3. Micropropagation
4. Somatic Cell Genetics 4.1. Somaclonal variation
4.2. Generation of haploids and double haploids
4.3. Protoplast isolation and culture
4.4. Genetic transformation 4.4.1. Breeding objectives
4.4.2. Protocol
4.5. Cryopreservation
5. Conclusions
Acknowledgements
References
Страница 153
1. Introduction 1.1. Botany and history
1.2. Breeding and genetics
2. Molecular Genetics
2.1. Genomics
3. Micropropagation
4. Somatic Cell Genetics 4.1. Regeneration 4.1.1. Somatic embryogenesis
4.1.2. Haploid recovery
4.1.3. Protoplast isolation and culture
4.2. Genetic manipulation 4.2.1. Mutation induction and somaclonal variation
4.2.2. Genetic transformation
4.3. Cryopreservation
5. Conclusions
Acknowledgements
References
Страница 168
1. Introduction 1.1. Botany and history
1.2. Breeding and genetics
2. Molecular Genetics
2.1. Molecular markers
2.2. Genetic linkage maps
2.3. Genomics
3. Micropropagation
4. Somatic Cell Genetics 4.1. Regeneration 4.1.1. Somatic embryogenesis
4.1.2. Organogenesis
4.1.3. Haploid recovery
4.1.4. Protoplast isolation and culture
4.2. Genetic manipulation 4.2.1. Mutation induction and somaclonal variation
4.2.2. Genetic transformation
4.3. Medium-term storage and cryopreservation
5. Conclusions
References
Страница 185
1. Introduction 1.1. Botany and history
1.2. Breeding and genetics
1.2.1. Major breeding objectives
1.2.2. Pest and disease tolerance
2. Molecular Genetics 2.1. Marker-assisted selection
2.2. Gene cloning
2.3. Genomics
3. Micropropagation
4. Micrografting
5. Somatic Cell Genetics 5.1. Regeneration 5.1.1. Somatic embryogenesis
5.1.2. Organogenesis
5.1.3. Haploid recovery
5.1.4. Triploid recovery
5.1.5. Protoplast isolation and culture
5.2. Genetic manipulation 5.2.1. Mutation induction and somaclonal variation
5.2.2. Genetic transformation
5.3. Cryopreservation
6. Conclusions
Acknowledgements
References
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