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5.1.3. Haploid recovery

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Germanà (2006), Fraser and Harvey (1986) and Oliveira and Fraser (2005) reported that haploid recovery has been unsuccessful in Actinidia. Trihaploid plants have been recovered in ‘Hayward’ by parthenogenic induction though pollination with gamma-irradiated pollen (Pandey et al., 1990; Chalak and Legave, 1996, 1997). After pollination of ‘Hayward’ with irradiated pollen from a male plant (‘D uno’), four trihaploid plants and a double trihaploid plant were recovered (Chat et al., 2003). Chromosome doubling was also obtained following treatment with oryzalin and adventitious regeneration (Chalak and Legave, 1996). As A. chinensis var. deliciosa may be an allopolyploid, the double trihaploids obtained by chromosome doubling (spontaneous or induced) are not necessarily homozygous for a number of genetic loci (Oliveira and Fraser, 2005). Chat et al. (2003) analysed the trihaploid and the double trihaploid plants and determined that the chloroplast genome of the male pollen donor plant was transmitted to the trihaploid progeny and that all the nuclear SSR loci tested were of maternal origin in the progeny.

Biotechnology of Fruit and Nut Crops

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