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3.1.3. Protoplast isolation and culture

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There has been a single attempt to develop a protocol for recovery of somatic hybrids (Haibou and Kovoor, 1981). They reported the isolation of protoplasts from immature inflorescence rachillae tissue and the production of micro-calluses from those protoplasts. A low rate of micro-callus proliferation occurred, and plantlet regeneration was not achieved.

Biotechnology of Fruit and Nut Crops

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