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3.2. Genetic manipulation 3.2.1. Mutation induction and somaclonal variation

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Although somaclonal variation is common in oil palm, it has not yet been identified in coconut. In a recent review (Sáenz-Carbonell et al., 2016), no physiological differences, e.g. germination rate, number of pollen grains, number of female flowers, etc., between somatic embryo and sexually produced plants have been observed. A molecular analysis based on AFLP markers also indicated that no significant change in genetic make-up of somatic embryo-derived coconut plants was generated (S. Fletcher, unpublished data). Sisunandar et al. (2010a) reported no genetic or epigenetic change in plantlets that had been regenerated following cryopreservation. New molecular tools, i.e. DNA sequencing, should be used to examine for significant genetic changes that may occur during the embryogenic pathway.

Biotechnology of Fruit and Nut Crops

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