Читать книгу Biotechnology of Fruit and Nut Crops - Группа авторов - Страница 40

Philip (1984) first reported organogenesis and plantlet recovery in cashew. Laxmi Sita (1989) also reported differentiation of leafy shoots from callus. Morphogenesis was achieved from nodular structures on cotyledons on Schenk and Hildebrandt (SH) (1972) medium with NAA and BA (Falcone and Leva, 1990). Roots were formed from cotyledonary explants but did not have vascular connection with the shoots (Sy et al., 1991). Enlargement of the resin ducts in the region of cotyledonary nodes occurred when they were cultured on MS medium with 44.4 μM BA and 14.6 mM maltose. These enlargements form adventitious buds from the tissues lining the resin ducts (D’Silva and D’Souza, 1992). Boggetti et al. (1999) studied factors affecting shoot development from nodes and regeneration of shoots and somatic embryos from immature cotyledons. Ananthakrishnan et al. (2002) have obtained adventitious shoots from cotyledon explants.