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2.2. Gene mapping

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Several genetic linkage maps have been developed in mango using different molecular markers. Chunwongse et al. (2000) constructed a genetic map with 31 F1 plants from a cross between ‘Alphonso’ and ‘Palmer’ using 197 RFLPs and 650 AFLPs, with 63 linkage groups for ‘Alphonso’, with an average of 3.3 markers per linkage group, and 59 linkage groups for ‘Palmer’, with an average of 4.2 markers per linkage group. Kashkush et al. (2001) published a genetic map with 13 linkage groups that involved seedlings of a cross between ‘Tommy Atkins’ and ‘Keitt’ using 34 AFLPs on 29 F1 individuals. Fang et al. (2003) used 191 AFLPs with 60 F1 individuals from a cross between ‘Keitt’ and ‘Tommy Atkins’ with 15 linkage groups. Chunwongse et al. (2015) used 9 SSRs and 67 RFLPs markers with 31 F1 plants from a cross between ‘Alphonso’ and ‘Palmer’ obtaining 29 linkage groups. However, all those linkage maps were insufficiently resolved to be used for marker-assisted selection or to develop a saturated map. More recently, Luo et al. (2016) used high-throughput sequencing to develop 13,844 single nucleotide polymorphisms (SNPs) that were used with 173 F1 plants from a cross between ‘Jin-Hwang’ and ‘Irwin’ to develop a linkage map with 20 linkage groups. Kuhn et al. (2017) produced a mango consensus genetic map based upon individual maps from seven F1 hybrid populations. This is the first such map that can be utilized for breeding and selecting new mango cultivars. Associations of SNP markers with blush intensity, beak shape and ground skin colour were significant.

Several groups have presented transcriptomes from different mango tissues. Thus, Azim et al. (2014) characterized the mango leaf transcriptome from ‘Langra’, identifying genes involved in biosynthetic pathways, plant hormone signal transduction, proteolytic enzymes and stress response. Wu et al. (2014) analysed the fruit transcriptome of ‘Zill’; Luria et al. (2014), the fruit pericarp transcriptome from ‘Shelly’ and Dautt-Castro et al. (2015), the fruit mesocarp transcriptome from ‘Kent’, expanding initial results of Pandit et al. (2010). Recently Kuhn et al. (2016) have performed a thorough transcriptome analysis from six different tissues (leaves, flowers, exocarp, mesocarp, seedcoat and seed) and from fruit tissues at different developmental stages in ‘Tommy Atkins’.

Using next-generation sequencing technologies, Sherman et al. (2015) identified a total of 332,016 SNPs and 1903 SSRs and used a subset of 293 SNPs to analyse genetic diversity in the Israeli mango collection. Kuhn et al. (2016) reported the development of approximately 400,000 SNPs after RNA analysis of 24 mango cultivars.

Dillon et al. (2014) identified 25 mango EST-SSRs linked to candidate genes involved with development, fruit colour and flavour and stress response that could be used for the study of diversity.

Different research groups are working on sequencing the mango genome. Examples include a draft genome of ‘Amrapali’ in India (Singh et al., 2014), sequencing of ‘Kensington Pride’ in Australia (Innes et al., 2015) and of ‘Tommy Atkins’ in the USA and Israel (Kuhn et al., 2018).

Biotechnology of Fruit and Nut Crops

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