Читать книгу Biotechnology of Fruit and Nut Crops - Группа авторов - Страница 60

The morphogenic potential of mango cotyledon explants was demonstrated by Rao et al. (1982), who described the induction of adventitious roots from callus that had been initiated on MS medium supplemented with naphthaleneacetic acid (NAA) and kinetin. Adventitious shoot induction was not observed. Raghuvanshi and Srivastava (1995) successfully initiated shoot-forming callus from fully expanded, young ‘Amrapali’ (monoembryonic) leaves. Leaf pieces were cultured in 100 ml liquid MS medium supplemented with 0.05% (v/v) PVP at 2 h intervals for c.24 h to fresh antioxidant solution and then cultured on induction medium, i.e. MS medium supplemented with 13.0 μM kinetin and 1.1 μM IAA. Induction medium was also the optimum formulation for stimulating multiple shoot formation. Individual mango shoots were rooted on MS medium with 9.8 μM IBA. Cultures were maintained at 25°C with a 16 h photoperiod provided by cool white fluorescent lights (50–70 μmol/m/s).