Читать книгу Biotechnology of Fruit and Nut Crops - Группа авторов - Страница 95

Nair et al. (1983) described haploid induction from cultured anthers of A. squamosa. Dissected anthers from 1 to 1.5 cm flower buds, containing mid-uninucleate and uninucleate pollen grains, formed callus on Nitsch medium supplemented with 28.6 μM IAA. Callus formation from anthers required a preliminary incubation period of 7 days in darkness. Regeneration of single shoots from callus occurred on Nitsch medium supplemented with 8.9 μM BA and 5.4 μM NAA. Multiple shoot development occurred on Nitsch medium supplemented with 8.9 μM BA and 0.6 μM IAA. The haploid number of regenerated plantlets (n = x = 7) was verified.