Читать книгу Biotechnology of Fruit and Nut Crops - Группа авторов - Страница 96

A ruminate type of endosperm is characteristic of the Annonaceae. Attempts to regenerate triploid (3n = 3x = 21) plants from endosperm tissue have had limited success. Shoots or roots, but not complete plantlets, have been regenerated from mature endosperm of A. squamosa (Nair et al., 1986). Endosperm was removed from sterilized mature seeds and explanted 2–4 days after germination. Seeds were pretreated for 1 h with 0.3 mM gibberellic acid (GA₃) and incubated on White’s basal medium (White, 1963) supplemented with 2.9 μM GA₃, 5.4 μM NAA, 0.9 μM BA and 0.5 μM kinetin. Triploid shoots were induced from endosperm callus on Nitsch medium supplemented with 8.9 μM BA and 2.7 μM NAA, and triploid root induction occurred on medium supplemented with 28.6 μM IAA.