Читать книгу The SAGE Encyclopedia of Stem Cell Research - Группа авторов - Страница 297

The midsubstance of patellar tendons is removed from the knee of human donors or harvested from bodies donated to research. The tissue samples are stored in phosphate buffer saline (PBS), after which they are cut into small pieces. The tiny pieces of tissue are treated with type 1 collagenase to digest the tissues, and then strained, using a cell strainer, to produce a suspension of single cells.