Читать книгу The SAGE Encyclopedia of Stem Cell Research - Группа авторов - Страница 299

Human tendon stem cells that are grown in culture have to be prepared before they are introduced into the affected area in the patient. The cells from the second day of culture are added to a 24-well culture dish using a seeding density at 6 × 10⁴ cells per well. A hypoxic condition is maintained at 5 percent oxygen at 37 degrees C. The cells are allowed to grow for one week, after which the cells are removed from culture and mixed with 0.5 ml 5 percent engineered tendon matrix. It has been found that human tendon stem cells that are implanted with engineered tendon matrix are better at developing into tendons at the site of injury.

The stem cells that are inserted into the site of injury show greater differentiation when they are grown in hypoxic conditions, which is indicative that engineered tendon matrix should also be developed in hypoxic conditions.