Читать книгу The SAGE Encyclopedia of Stem Cell Research - Группа авторов - Страница 298

The isolated cells are then added to a six-well culture plate that contains 3 ml Dulbecco’s Modified Eagle Medium 20 percent fetal bovine serum, 10 U/ml penicillin, 100 αg/ml streptomycin, and 2 mM l-glutamine. An atmosphere of 5 percent oxygen is maintained throughout the process of cell culture, as tendons are present in the body in a hypoxic condition, so tendon stem cells grow better in hypoxic conditions rather than at a normal oxygen level.

On the second day of culture, the cells that do not adhere to the walls of the culture flask are removed by adding PBS. After seven to 10 days, the cells in culture are treated with trypsin to remove them from the flask. Reculture is performed by first placing the culture flask with medium under hypoxic conditions for 30 minutes. The cell population is counted on days 1, 2, 6 and 12.