Читать книгу Diagnostics and Therapy in Veterinary Dermatology - Группа авторов - Страница 47

Serologic assays detect antigens or antibodies in bodily fluids, particularly serum. Measurement of the immune complexes formed when antibody molecules bind reversibly to antigens is the basis of serology. Antigen tests used in the diagnosis of infectious diseases allow for early detection of organisms. Interestingly, serologic assays are used to measure any “antigen” of interest, including cytokines, white blood cells, proteins, and cellular by‐products. In contrast to antigen tests, antibody tests demonstrate the immunologic response to certain antigens by measuring serum antibodies against specific antigens not present or easily isolated from the tissue. There are three major techniques used for serologic assays: primary binding, secondary binding, and tertiary binding (Tizard 2013).