Читать книгу Diagnostics and Therapy in Veterinary Dermatology - Группа авторов - Страница 57

Prior to whole‐genome sequencing made possible by PCR technology, pulsed‐field gel electrophoresis (PFGE) was the gold standard for fingerprinting DNA. This technique uses enzymes to digest whole DNA from microbes or mammalian cells into large fragments that are separated according to their size using a fluctuating electric field (Herschleb et al. 2007). “Fingerprinting” comes from the unique pattern the DNA fragments create for each organism. PFGE can separate very large fragments of DNA, unlike other types of conventional DNA electrophoresis. The unique DNA fingerprint is a form of genotyping that can be used to discriminate among different strains of an organism (e.g. for identifying different strains of Staphylococcus pseudintermedius grown on aerobic culture). DNA fragments are separated and extracted for further evaluation using other laboratory techniques. This technique is mainly used for research at this time.