Читать книгу Diagnostics and Therapy in Veterinary Dermatology - Группа авторов - Страница 51

IHC staining is an ELISA‐based technique commonly used in veterinary medicine to detect antigens within tissue, specifically formalin‐fixed tissue. IHC has many applications as a diagnostic modality, since it detects molecular markers and proteins in cells that identify cell types and determines where in the cell the antigen of interest exists. For example, a chromogenic assay using rabbit anti‐Pythium antibodies is used to detect Pythium sp. hyphae within formalin‐fixed tissue from dogs (Brown et al. 1988). Colorimetric (chromogenic) and fluorescent assays are most commonly used for visualizing the antigen–antibody interaction in the tissue. The detection antibodies used may be monoclonal or polyclonal as described earlier, which can influence the binding sensitivity and specificity of the assay.