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Flow Cytometry

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The physical and chemical characteristics of individual cells and particles suspended in a fluid can be analyzed using flow cytometry. This methodology uses a laser illuminating beam that passes over the molecules within the liquid sample as they advance single file through the flow chamber (Pedreira et al. 2013). This technique allows for rapid, objective, and quantitative evaluation of cells, even when present in small numbers within the sample. Flow cytometry uses light scatter and absorption to measure individual cells and has the added ability to sort cells based on these characteristics into separate collection tubes for future experiments. Flow cytometry is a useful tool for the diagnosis and study of hematologic neoplasia in veterinary medicine (Meichner et al. 2020).

Diagnostics and Therapy in Veterinary Dermatology

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