Читать книгу Pathology of Genetically Engineered and Other Mutant Mice - Группа авторов - Страница 47

While one can read a paper and contact the corresponding author to try to obtain mice to develop a colony for research, there are always concerns about real availability, time to get the mice, genetic quality (is the strain really what it is claimed to be?), pathogen status, and many other variables. Fortunately, a large number of commercial and nonprofit institutions have set up repositories to obtain, standardize, and distribute mice world‐wide. Some are listed in Table 2.4. Information provided on the websites can be quite detailed and specific while others can be limited to the degree that sometimes the specific mutation in the gene is not known. However, careful study of the literature provides a lot of the information needed to make an informed decision.

An alternative approach is to do your initial searches on MGI (http://www.informatics.jax.org, described above). This website is not a mouse repository. However, if you look up your gene of interest (under the header Genes and then search for the gene of interest in the Genes and Marker Query) you end up at a Gene Detail page. On the lower right side is a category “All mutations and alleles” with a number. If you click on that (or the specific type listed below) you will get a listing of all published alleles with the correct (at the time of viewing) gene and allele symbol with a short summary. Click on the link to the allele of interest and you will get more information on that allele. At the bottom of the page, just above the reference list, is a section “Find Mice IMSR”. Click on that link and it will take you to the International Mouse Strain Resource (IMSR) or you can go to their site directly from the start (http://www.findmice.org). This will tell you where in the world the mice are currently available from.