Читать книгу Pathology of Genetically Engineered and Other Mutant Mice - Группа авторов - Страница 57

Another variation of RI lines is the Collaborative Cross. This is a complex set of crosses using eight different inbred strains that represent a great deal of genetic diversity [17, 18]. The goal was to create over 1000 new inbred strains. Due to reproductive failure and low viability of many strains, the current goal is to create 200 strains. These mice present with great phenotypic variability, a reflection of the great genetic diversity. These can be used to screen for specific phenotypes [19, 20]. When a large enough cohort are examined, phenotyped, and genotyped using the Mouse Universal Genotyping Array (Single Nucleotide Polymorphism [SNP] Array), it is possible to identify candidate gene regions and even the gene(s) responsible for the phenotype [21].

Collaborative Cross mice are designated by CC for Collaborative Cross, the line number, forward slash, and the lab that created it followed by the laboratory that currently maintains it. For example, CC001/UncJ (stock number 021238) is line number one, generated at the University of North Carolina, and currently maintained and distributed by The Jackson Laboratory.