Читать книгу Pathology of Genetically Engineered and Other Mutant Mice - Группа авторов - Страница 67

There are three basic types of genetically engineered mice: transgenic, targeted, and endonuclease‐mediated. Different nomenclature is used to distinguish these.

Transgenic mice can be created to overexpress a gene, express a gene not normally found in mice (green fluorescent protein [GFP] from jellyfish), conditionally express genes, and many other engineered approaches not found normally in nature.

Transgenic nomenclature can be complex, especially as some transgenes still use older formats (Table 3.13). All transgenes begin with Tg followed by information in parentheses then a founder line number and lab code. If a single gene driven by its own promoter is inserted, then the information in the parentheses is simply that gene symbol, not italicized. The information in the parentheses begins with the promoter, a hyphen, then information about the expressed sequence(s). For example, C57BL/6J‐Tg(Mt1‐Tnfsf4)1Pgn/Pgn has a transgenic insertion of mouse tumor necrosis factor (ligand) superfamily, member 4 (Tnfsf4) gene under the control of the metallothionine promoter coisogenic on the C57BL/6J background and is the first transgenic line with these components made by Dr. Beverly Paigen [44].

Table 3.13 Nomenclature for transgenic mice.

FVB/NJ‐Tg(KRT14‐HPV18E7)CSun/SunFVB/NJ = genetic background of the strainTg = Transgene insertionKRT14 = Human KRT14 promoterHPV18E7 = Human papillomavirus 18 E7 geneC = transgenic lineSun = strain lab code, John P. Sundberg

B6N.Cg‐Tg(KRT14‐cre)1Amc/JB6N = Congenic host strain carrying the allele, C57BL/6NCg = donor strain is not known (C57BL/6 x CBA)F1Tg = Transgene insertionKRT14 = Human keratin 14 genecre = cre‐recombinase expressing1Amc = line 1, Andrew P. McMahonJ = strain lab code, The Jackson Laboratory