Читать книгу Snyder and Champness Molecular Genetics of Bacteria - Tina M. Henkin - Страница 120

Dideoxynucleotides are similar to the normal deoxynucleotide precursors, except that they lack a hydroxyl group on the 3′ carbon of the deoxynucleotide. Consequently, they can be incorporated into DNA, but then replication stops because they cannot link up with the next deoxynucleotide. These compounds are not useful antibacterial agents, probably because they are not phosphorylated well in bacterial cells. However, this property of prematurely terminating replication has made them the basis for one of the first methods for DNA sequencing (see chapter 13).