Читать книгу Snyder and Champness Molecular Genetics of Bacteria - Tina M. Henkin - Страница 123

5-Bromodeoxyuridine (BUdR) is similar to thymidine and is efficiently phosphorylated and incorporated in its place into DNA. However, BUdR incorporated into DNA often mispairs and increases replication errors. DNA containing BUdR is also more sensitive to some wavelengths of ultraviolet (UV) light (which makes BUdR useful in enrichment schemes for isolating mutants [see chapter 3]). Moreover, DNA containing BUdR has a different density from DNA exclusively containing thymidine (another feature of BUdR that is useful in experiments).