Читать книгу Snyder and Champness Molecular Genetics of Bacteria - Tina M. Henkin - Страница 25

The ability of some types of bacteria to form colonies through the multiplication of individual bacteria on plates allows colony purification of bacterial strains and mutants. If a mixture of bacteria containing different mutants or strains is placed on an agar plate, individual mutant bacteria or strains in the population each multiply to form colonies. However, these colonies may be too close together to be separable or may still contain a mixture of different strains of the bacterium. If the colonies are picked and the bacteria are diluted before replating, discrete colonies that result from the multiplication of individual bacteria may appear. No matter how crowded the bacteria were on the original plate, a pure strain of the bacterium can be isolated in one or a few steps of colony purification.