Читать книгу Snyder and Champness Molecular Genetics of Bacteria - Tina M. Henkin - Страница 28

Most types of organisms must be continuously propagated; otherwise, they age and die. Propagating organisms requires continuous transfers and replenishing of the food supply, which can be very time-consuming. However, many types of bacteria can be stored in a dormant state and therefore do not need to be continuously propagated. The conditions used for storage depend on the type of bacteria. Some bacteria sporulate and so can be stored as dormant spores. Others can be stored by being frozen in glycerol or by being dried. Storing organisms in a dormant state is particularly convenient for genetic experiments, which often require the accumulation of large numbers of mutants and other strains. The strains remain dormant until the cells are needed, at which time they can be revived. Organisms that are continually propagated will also accumulate mutations, a problem that is eliminated with the proper storage of bacteria.