Читать книгу Practical Equine Dermatology - David H. Lloyd - Страница 16

Useful to determine whether the lesions of alopecia or hypotrichosis are due to self‐inflicted damage (fractured hair shafts, split ends) indicating that the condition is pruritic, or due to abnormal hair growth (absence of anagen roots, abnormal catagen roots), and to examine for dermatophytes and for parasite eggs.

 Choose fresh, unmedicated lesions.

 For suspected dermatophytosis, where cultures are required, first lightly clean the areas to be sampled with 70% alcohol (to reduce contaminant organisms).

 Tissue or epilation forceps can be used to grasp gently and pull out hairs from the periphery of the lesion.

 Samples for microscopy can be placed on adhesive tape wrapped around a microscope slide and mounted in a drop of liquid paraffin just prior to examination.

 Samples for fungal culture submission should be held in paper or sterile, non‐airtight containers to prevent a humid environment that might support the growth of saprophytic organisms.