Читать книгу Practical Equine Dermatology - David H. Lloyd - Страница 17

Useful for cytological examination looking for bacterial organisms (particularly Dermatophilus) and for submission for fungal and bacterial culture.

 Choose a fresh, unmedicated lesion.

 Impression smears of the underside of freshly removed crusts, stained with a rapid Romanowsky‐type stain (e.g. Diff‐Quik, Hemacolor, Rapi‐Diff, Speedy‐Diff) or Gram’s stain, can provide a quick method of diagnosis for dermatophilosis.

 Crusts can be collected and held in paper envelopes or sterile containers for transport to the laboratory.

 Dried crusts can be emulsified in a drop of sterile saline on a slide, warmed to allow rehydration of material, prior to air‐drying and fixing (heat fixation for Gram’s stain, methanol/ethanol fixation for rapid‐differentiating Romanowsky‐type stains) for cytological examination and identification of bacterial and fungal organisms.