Читать книгу Practical Equine Dermatology - David H. Lloyd - Страница 21

From fresh, exudative, crusted, excoriated, or pustular lesions, a direct impression smear can be made for cytological examination and for microorganisms.

 Press a glass slide against the concave undersurface of a removed exudative crust, or against the surface of a freshly exposed lesion.

 For an intact pustule, gently break the overlying skin with a 25 g needle and press a clean glass slide to the ruptured lesion, or purulent material may be collected in the needle bevel and then transferred to the glass slide.

 For lesions at sites where it is difficult to apply a slide directly, material can be collected with a dry swab and then rolled onto a glass slide.

 Air‐dry the slide and store in a slide box prior to heatfixing (for Gram staining) or immersion in methanol (for Romanowsky‐type staining).