Читать книгу Practical Equine Dermatology - David H. Lloyd - Страница 20

An alternative method for obtaining surface material, including Oxyuris equi eggs, surface‐living ectoparasites, hair fragments, exfoliated cellular material, and surface microorganisms for direct microscopical examination or after staining; it is less traumatic and avoids the risk of injury associated with skin scrapes. This technique is particularly useful for identification of chorioptic mange mites which are highly motile, but also allows detection of other pathogens including dermatophytes and yeasts.

 A piece of clear adhesive tape (e.g. 3M Scotch Crystal, Sellotape Clear) is applied to the lesional area 3–4 times (Figure 1.5).

 Tape is applied (sticky side down) to a microscope slide over a drop of liquid paraffin for direct examination or over a drop of blue dye from a rapid Romanowsky‐type stain kit.

 Excessive mounting medium or stain is removed by wiping with soft paper towel prior to microscopical examination.

Figure 1.5 Surface adhesive tape sampling.