Читать книгу Practical Equine Dermatology - David H. Lloyd - Страница 22

For older, crusted lesions this technique enables microscopical examination of dried exudate.

 Representative sample of crust is placed on a glass slide with a few drops of normal saline.

 Material is finely chopped and macerated with a scalpel blade.

 Slide is left in a warm place for 20–30 min to allow rehydration of cellular material.

 Any large clumps of debris are gently removed prior to thorough drying and heat fixing of the remaining suspension prior to staining with a rapid Romanowsky‐type stain kit.