Читать книгу Practical Equine Dermatology - David H. Lloyd - Страница 24

This technique is used for sampling nodules, masses, and enlarged superficial lymph nodes.

 A 20–22 g needle can be used, with or without a 5 ml syringe. The area to be aspirated should be carefully cleaned and disinfected.

 The needle is inserted into the nodule (Figure 1.6), mass, or lymph node and used to probe the tissue in several places, initially without aspirating, and subsequently whilst gently aspirating.

 The needle is withdrawn from the tissue and detached from the syringe, which is then filled with a small amount of air, reattached to the needle, and the sample expressed directly onto a clean slide for cytology or onto a swab for culture. A second slide is placed over the sample to spread the material. The slides are then separated gently to avoid damaging cells and air‐dried prior to staining for microscopical examination.