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Heavy metals also have negative effects on Soil Organic Matter (SOM) as it decreased up to 1% in those soils contaminated with Zn and Cu by hindering the microbial activity and plant growth [36, 37]. In another study, HMs showed a positive relationship with SOM because an adverse HM effect on soil microbes appeared to increase the accumulation of organic matter but the relationship was negative in terms of soil respiration and microbial biomass since the bacteria were less effective in mineralizing SOM under these conditions [38, 39]. Microbial activities such as respiration, C and N mineralization, biological N2 fixation, and soil enzymes were assessed for this [40]. SOM degradation leads to changes in the soil, these are assessed by their change with regards to Zn, Pb, and Cu that caused a decrease in SOM after soil has received long‐term application of sewage sludge [41].