Читать книгу Biomolecular Engineering Solutions for Renewable Specialty Chemicals - Группа авторов - Страница 19

Alkaline phosphatase prevents self‐ligation of DNA molecule (vectors and gene of interest) in genetic engineering experiment by dephosphorylating phosphate group on 3′ end of the DNA molecule. It is extracted from E. coli or calf intestine. The enzymes catalyze the hydrolysis of monoesters in phosphoric acid which can moreover catalyze a trans‐phosphorylation reaction with large concentrations of phosphate acceptors. It can be used to prevent self‐ligation of vectors in the cloning experiments because alkaline phosphate‐treated DNA fragments lack the 5′‐phosphophate in the terminal, required for the actions of DNA ligases (Tamás et al., 2002).