Читать книгу Biomolecular Engineering Solutions for Renewable Specialty Chemicals - Группа авторов - Страница 21

Terminal deoxy‐nucleotidyl transferase [Terminal transferase (TdT)] has the capability to transfer or add polynucleotide in 3′ terminus of the DNA molecule. It is extracted from calf thymus. TdT is a template impartial polymerase that catalyzes the addition of deoxynucleotides to the 3′ hydroxyl terminus of DNA molecules with none template strand. In rDT, blunt‐ended DNA strands are required every now and then to make it cohesive via the addition of nucleotides at 3′ of one strand (Greider and Blackburn, 1985)