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3.3.1.2 Lignocellulosic Biomass (Second-Generation - 2G)
ОглавлениеLignocellulosic biomass, a second-generation (2G) feedstock, is an abundantly available agricultural residue that generates cellulosic ethanol as renewable biomass and ethanol fuel [31] (Table 3.1). Limitation behind this type of biomass conversion includes expensive pretreatment and hydrolysis (specifically via enzymes), lack of pentose and hexose simultaneously saccharifying potential microorganisms. Nowadays, the Indian government wants to establish ethanol production from the non-feedstock substrate (Figure 3.6) rather than first-generation biomass like sugarcane. Second-generation biomass involved in 2G ethanol production in India includes rice straw (47%), cotton stalk (11%), and municipal solid waste (MSW), wheat stalk, soya stalk, bagasse, maize, bamboo, and corn cob (each 6%) utilized as a renewable resource [33].
Figure 3.5 Sustainable renewable energy production from various biomass resources in states of India in 2020 [4].
Figure 3.6 Biomass utilization in India (2019-20) for production of second-generation ethanol (2G) [33].
Table 3.1 List of first-generation (1G) and second-generation (2G) biomass, the process of conversion and biofuel products [27, 32, 34–39].
Types of biomass | Biomass | Process of conversion | Biofuel products | Reactor/fermentation process | Reference |
Sugar and starch-based biomass (first-generation - 1G) | cassava bagasse from the cassava starch industry with steep corn liquor | Dilute acid pretreatment and enzyme hydrolysis (glucoamylases and cellulases) | n-butanol | fibrous-bed bioreactor | [32] |
Sweet potato with dairy cattle manure | Anaerobic co-digestion | Biogas | Semi-continuous digesters | [34] | |
sugarcane molasses | Anaerobic fermentation by Bacillus species (mesophilic condition) | Biofuel (ethanol, butyric acid, acetic acid, and lactic acid) | - | [35] | |
Lignocellulosic biomass (Second generation - 2G) | Paddy straw supplemented with fruit waste | Alkali treatment with 2% NaOH/ Hydrolysis by Commercial cellulase enzymes (Palkonol and Palkosoft)/ fermentation by Saccharomyces cerevisiae HAU-1 and Candida sp. | Bioethanol | SSCF | [27] |
Cassava stem, leaves, and peels | Microwave (MW)assisted alkali pretreatment (300 W, 7 min)/Hydrolysis by triple enzyme cocktail with detoxification/ fermentation by Saccharomyces cerevisiae | Bioethanol | SHF | [36] | |
Napier grass | Hydrolysis by Aspergillus niger and Trichoderma reesei/fermented by Zymomonas mobilis | Bioethanol | SSCF | [37] | |
Sugarcane trash | Crude glycerol assisted transition metal and alkali pre-treatment/ hydrolysis by commercial cellulase/fermented by Saccharomyces cerevisiae | Bioethanol | - | [38] | |
Taro waste (TW) | Hydrolysis by α- amylase from Bacillus licheniformis amyloglucosidase from Aspergillus niger/fermented by Kluyveromyces marxianus K21 | Bioethanol | SSF/SHF | [39] |
Table 3.2 Agencies involved in the production of second-generation ethanol (2G) 100 kilo Liter/day in India (2019-20) by use of rice straw [33].
Companies | Cities | States |
IOCL | Panipat | Haryana |
Gorakhpur | Uttar Pradesh | |
BPCL | Bargarh | Odisha |
Bhandara | Maharashtra | |
HPCL | Bhatinda | Punjab |
Badaun | Uttar Pradesh | |
Muzzafarpur | Bihar | |
East and West Godavari | Arunachal Pradesh |
Several agencies such as IOCL, BPCL, HPCL, NRL, and MRPL in India are involved in 2G ethanol production (Table 3.2). Among these agencies, IOCL, BPCL, and HPCL in India’s different states produce a maximum 2G ethanol of 100 Kilo liter/day from rice straw [33].