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3.3.1.4 Genetically Engineered Biomass (Fourth-Generation)

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The metabolic engineering of microorganisms with elevated organic matter explored for the development of biofuels forms that become the basis for developing fourth-generation biofuels [6]. The third and fourth generations of biofuel production include organic matter technology into biofuels (Table 3.3). Huang et al. [48] evaluated that genetically engineered OsSEX4-knockdown transgenic rice plants with starch accumulation higher than the wildtype forms as a potential source to produce bioethanol. OsSEX4 plays a vital role in the degradation of transitory starch; thus, it increases the plant’s starch content by knockdown. Genetically modified biomass produces bioethanol 50% higher than the wild-type straw in a vertical mass-flow type bioreactor (two-tank type bioreactor) by simultaneous saccharification and fermentation (SSF) [48, 52].

Renewable Energy for Sustainable Growth Assessment

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