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Five milliliters of venous whole blood was drawn by disposable syringe. The blood sample was transferred immediately into a labeled container and stored in an ultra‐freezer at −20 °C to avoid any microbial growth. Blood samples should be collected in plastic containers prior to pretreatment and kept in a 20% nitric acid solution for 24 hours, properly rinsed with deionized water, and dried. The empty weight of all containers were taken before being stored in a plastic covering. The total weight of blood with containers was taken at room temperature. Subtracting the empty weight of the container gave the weight of the fresh sample. The samples were dried in an oven at 60–70 °C for four to six days. The dried samples were powdered in a carbide mortar with the help of a pestle and preserved in desiccators until subsequent analysis [15].