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1.4.3 Particle Size and Surface Area Characterization

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Various techniques can calculate the size and surface area of the NPs. These include dispersing SEM, TEM, XRD, AFM, and dynamic light scattering (DLS). It is possible to increase the particle size of SEM, TEM, XRD, and AFM, but the zeta theoretical analyser/DLS should be used to find the NP size at a weak stage. In one study, DLS was used to analyse silica NP size differences while consuming serum proteins. With the acquisition of the protein layer, the findings showed that the size increased. However, in the case of aggregation and hydrophilicity, DLS might prove incapable of accurate measurement, so in that case, we should focus on the high‐resolution technique of differential centrifugal sedimentation [58–60].

Nano-Technological Intervention in Agricultural Productivity

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