Читать книгу Transfusion Medicine - Jeffrey McCullough - Страница 178

Granulocytes have a life span in circulation of only a few hours, so storage of granulocytes as part of a routine blood bank operation is difficult. Granulocytes retain bactericidal capacity and metabolic activity related to phagocytosis and bacterial killing for 1–3 days with storage at refrigerator temperatures, although chemotactic response declines by 30–50% after 24 hours [88–91]. Studies using ¹¹¹In‐labeled granulocytes showed that storage of granulocytes between 1 and 6°C for 24 hours was associated with a reduction in the percentage of transfused cells that circulated and about a 75% reduction in migration into a skin window [88], but storage at room temperature for 8 hours did not reduce the intravascular recovery, survival, or migration into a skin chamber [88]. In vivo recovery, survival, or migration was reduced further when granulocytes were stored longer than 8 hours at room temperature or for even 8 hours between 1 and 6°C. Thus, it appears that granulocytes can be stored for up to 8 hours at room temperature before transfusion.

Granulocyte concentrates from G‐CSF‐stimulated donors contain large numbers of granulocytes with increases in IL‐1B and IL‐8, and decreases in pH during storage [75]. Thus, storage of granulocyte concentrates obtained from G‐CSF‐stimulated donors is probably even less effective than these data indicated. It is recommended that granulocytes be transfused within a very few hours. AABB standards allow storage for up to 24 hours at 20–24°C [47].