Читать книгу Transfusion Medicine - Jeffrey McCullough - Страница 184

Large quantities of CD34+ cells can be collected from normal donors given G‐CSF for 5 days. G‐CSF doses of 7.5 or 10 mg/day provide a greater yield than 5 mg/day [115], but it is not clear whether there are statistically significant differences in the CD34+ cell yield between 7.5 and 10 mg/day. Because the donor side effects increase with increasing doses of G‐CSF, we recommend that donors be given 7.5 mg/kg/day of G‐CSF to mobilize peripheral blood CD34+ cells [115]. The composition of the PBSC component is shown in Tables 6.3 and 6.4. Most PBSC components contain only a small volume of red cells (mean, 7 mL) but a rather large total number of neutrophils and platelets [115]. This number of platelets is similar to the number of platelets ordinarily provided in a platelet transfusion, and if transfused fresh (i.e., allogeneic transplant) this may provide an additional benefit from the PBSC transfusion. The PBSC concentrate usually has a volume of about 200 mL and contains approximately 3 × 10¹⁰ MNCs and 4 × 10⁸ CD34+ cells.